holB [2017-12-07 13:26:53]

DNA polymerase III (delta subunit), part of the clamp-loader complex and the replisome

Locus: BSU00310

Isoelectric point: 6.33

Molecular weight: 37.47 kDa

Protein length: 329 aa

Gene length: 987 bp

Function: DNA replication

Product: DNA polymerase III (delta subunit)

Essential: yes

E.C.: 2.7.7.7

Synonyms: yaaS

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA replication
• Groups of genes → Essential genes

Gene

Coordinates: 40,665 → 41,654

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• Deoxynucleoside triphosphate DNA(n) = diphosphate DNA(n 1) (according to Swiss-Prot)
• required for bacteriophage SPP1 replication PubMed

Structure

• 1NJG (from E. coli, 36% identity) PubMed

Localization

• forms foci PubMed
• cytoplasm (homogeneous) PubMed

Expression and Regulation

Operons

• Genes: xpaC-yaaN-yaaO-tmk-darA-yaaR-holB-yaaT-yabA-trmN6-yazA-yabC
  Description: PubMed

• Genes: darA-yaaR-holB-yaaT-yabA-trmN6-yazA-yabC
  Description: PubMed

  Regulation

  • expressed during growth and the transition phase, expression is erduced in stationary phase PubMed

• Genes: tmk-darA-yaaR-holB-yaaT-yabA-trmN6-yazA-yabC
  Description: PubMed

Biological materials

Expression vector

• pGP1692: expression of Strep-holB-Strep in E. coli, in pGP172, available in Jörg Stülke's lab
• pGP1698: expression of holB-Strep in E. coli, in pGP574, available in Jörg Stülke's lab
• pGP1699: expression of holB in E. coli, in pGP574 (no tag due to a stop codon at the end of holB), available in Jörg Stülke's lab
• pGP2672: expression of holB-Strep in B. subtilis, in pGP380, suitable for SPINE, available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

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